Journal: Molecular medicine (Cambridge, Mass.)

Article Title: PI3K p85α/HIF-1α accelerates the development of pulmonary arterial hypertension by regulating fatty acid uptake and mitophagy.

doi: 10.1186/s10020-024-00975-9

Figure Lengend Snippet: Fig. 1 HIF-1α was closely associated with the Parkin/PINK1 axis and fatty acid metabolism-related factors in PAH patients. A Serum levels of HIF-1α were measured by ELISA. B Serum levels of Parkin and PINK1 were measured by ELISA. C Serum levels of FAS, ACC, and CD36 were measured by ELISA. *P < 0.05 vs. Normal. The statistical test was performed using unpaired Student’s t-test. D Spearman’s correlation analysis was performed to determine the correlation between HIF-1α, Parkin, PINK1, FAS, ACC, and CD36. *P < 0.05. ***P < 0.001. ****P < 0.0001

Article Snippet: The levels of HIF-1α, Parkin, PINK1, fatty acid synthetase (FAS), acetyl-CoA carboxylase (ACC), and CD36 in human or rat serum were measured according to the instructions of the following ELISA kits: Human HIF-1α ELISA kit (CSB-E12112h, CUSABIO Co., Ltd., Wuhan, China), Human Parkin ELISA Kit (JL11195, Shanghai Jianglai Industrial Limited by Share Ltd., Shanghai, China), Human PINK1 ELISA Kit (JL11175, Shanghai Jianglai Industrial Limited by Share Ltd.), Human/Rat FAS ELISA Kit (CSB-E04542h/CSB-E07324r, CUSABIO Co., Ltd.), Human/Rat ACC ELISA Kit (CSBEL001119HU, CUSABIO Co., Ltd./YJ059228, Shanghai Yuanju Biotechnology Center, Shanghai, China), and Human/Rat CD36 ELISA Kit (CSB-E10117h/CSBEL004927RA, CUSABIO Co., Ltd.).

Techniques: Enzyme-linked Immunosorbent Assay